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KMID : 0043319900130010069
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Archives of Pharmacal Research
1990 Volume.13 No. 1 p.69 ~ p.73
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Isolation and Partial Characterization of Rat LDH A-Genomic Sequences
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Lee MY
Yim SY/Lee SK
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Abstract
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As a part of the study to elucidate the mechanism by which the transcription of LDH A-gene is regulated by cAMP, we aimed to isolate rat LDH A gene and characterize cAMP-responsive element (CRE). We have screened 1.2 X 106 recombinant phages of rat Charon 4A genomic library and isolated 33 positive clones among which we identified 12 different LDH A gene-related clones. By the results of restriction enzyme mapping, Southern blotting, and nucleotide sequence analyses, we concluded that the 12 LDH A gene-related clones were intronless and frequently mutated LDH A-pseudogenes. In this report, we present the characteristic features of the 12 rat liver LDH A-pseudogenes.
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KEYWORD
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